Barbour was a high-level S.H.I.E.L.D. agent at the time surrounding the the collapse of the agency and the reemergence of HYDRA. He was one of five agents loyal to S.H.I.E.L.D. whom Agent Victoria Hand trusted and relied upon. He was present at The Hub when Agent Hand was defending it from the HYDRA threat within. Along with Agent Shaw, Barbour was tasked with finding Agents Triplett and Simmons, who were hiding in the lab.